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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9233^* Cite

Description

Immunofluorescence image of HeLa cell expressing a fusion protein of GFP-polypyrimidine tract binding protein showing the localization of the perinucleolar compartment (green area) at the periphery of the nucleolus (red region). The nuclear periphery is demarcated by immunolabeling of the nuclear lamina (blue).

Technical Details

See Huang et al. 1997, The Dynamic Organization of the Perinucleolar Compartment in the Cell Nucleus J. Cell Biol 137: 965-974.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: cervical carcinoma
Cell Line: HeLa
Cellular Component: nucleolus

Biological Context

Biological Process: RNA metabolic process

Attribution

Names: Sui Huang; Thomas J. Deerinck; Mark H. Ellisman; David L. Spector
Pubmed: 9166399

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9233
Archival Resource Key (ARK): ark:/b7295/w9cil9233

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; charge coupled device (CCD)
Parameters Imaged: fluorescence microscopy
Source of Contrast: fluorescence emission
Visualization Methods: TexasRed; EGFP

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	672px	40nm
Y	528px	40nm