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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:717*  Cite 
Description

X-ray analysis shows that the nucleated erythrocytes of the amphibian Necturus maculosus (Mud Puppy) contains more ordered chromatin than most nuclei. Here, chromatin fibers were isolated from N. maculosus erythrocyte nuclei using mild micrococcal nuclease digestion, fixed briefly with glutaraldehyde, applied to thin carbon films and negatively stained with aurothioglucose. ~30 nm fibers are seen, some exhibiting short regions with apparent structural order.

Biological Sources
NCBI Organism Classification
Necturus maculosus
Cell Type
nucleate erythrocyte
Cellular Component
nuclear chromatin
Biological Context
Biological Process
chromatin assembly
Molecular Function
DNA packaging
Attribution
Names
Woodcock, Chris
Pubmed
1757505
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL717
Archival Resource Key (ARK)
ark:/b7295/w9cil717
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
detection of electrons
Parameters Imaged
electron density
Source of Contrast
elastic scattering
Visualization Methods
other negative stain
Processing History
raw unprocessed data
Sample Preparation
Methods
unembedded tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 2035px 0.5nm
Y 3126px 0.5nm