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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40442^* Cite

Description

Transmission electron micrograph of a vacuole from a sertraline-treated drs2∆ mutant cell. This vacuole contains an undigested autophagosomes. This image is a high-magnification zoom of the cell in CIL40424 (25,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).

Technical Details

This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cell Type: drs2∆ mutant
Cellular Component: vacuole; autophgosome

Biological Context

Biological Process: organelle organization; sertraline treatment

Attribution

Names: Ethan Perlstein; Daniel Korostyshevsky
OTHER: Princeton EM Core Facility

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40442
Archival Resource Key (ARK): ark:/b7295/w9cil40442

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide
Processing History: unprocessed raw data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2328px	——
Y	2612px	——