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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40430^* Cite

Description

Transmission electron micrograph of an autophago-lysosome from a sertraline-treated prototrophic wildtype cell. (40,000X). This is part of an image group highlighting multilamellar structure, CIL: 40428-40430.

Technical Details

This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: autophagic vacuole; lysosome

Biological Context

Biological Process: organelle organization; sertraline treatment

Attribution

Names: Ethan Perlstein; Daniel Korostyshevsky
OTHER: Princeton EM Core Facility

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40430
Archival Resource Key (ARK): ark:/b7295/w9cil40430

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide
Processing History: unprocessed raw data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2148px	——
Y	2356px	——