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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40413^* Cite

Description

Transmission electron micrograph of a mature autophagosome from a BY4716 wildtype cell treated with 60µM sertraline. (40,000X). CIL 40414 is a similarly treated cell.

Technical Details

This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cell Type: BY4716
Cellular Component: autophagic vacuole

Biological Context

Biological Process: organelle organization; sertraline treatment

Attribution

Names: Ethan Perlstein; Daniel Korostyshevsky
OTHER: Princeton EM Core Facility

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40413
Archival Resource Key (ARK): ark:/b7295/w9cil40413

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide
Processing History: unprocessed raw data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2256px	——
Y	2456px	——