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Licensing
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40260*  Cite 
Description

Maximum intensity projection of Golgi impregnated pyramidal neurons in the neocortex of an adult mouse, imaged via serial block-face scanning electron microscopy. Contrast is reversed so that Golgi stain appears bright against the unstained background. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Perfused with 4% PFA, 0.1% glut. Postfixed 1 hour in same fix on ice. Left hemisphere cut into 2 mm thick coronal slabs. Tissue placed into 2.3% potassium dichromate / 0.4% OsO4 for 3 days at room temp. Tissue blotted with filter paper and then placed in 0.7% silver nitrate in dark for 3 days. Tissue was dehydrated, infiltrated and embedded in Durcupan in Beem capsules. Serial section images were gathered on an FEI Quanta 200 FEG SEM with Gatan 3 View microscope, magnification, 600.0 X, accelerating voltage, 2.0 kV.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
pyramidal cell
Cellular Component
neuronal cell body membrane
dendrite
axon
Attribution
Names
Mark Ellisman
Stephen Larson
Link
CCDB 8244
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40260
Archival Resource Key (ARK)
ark:/b7295/w9cil40260
Imaging
Image Type
recorded image
Image Mode
serial block face SEM (SBFSEM)
Parameters Imaged
back-scattered_electron imaging
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
X 435px ——