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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39747^* Cite

Description

(C) Overview of cluster of metaphase chromosomes from a Drosophila embryo visualized using fluorescence microscopy showing the DAPI (blue) and histone (green) fluorescence. (A) PALM image of region in lower box in (A). Red arrowheads point to filamentous structures. See Fig 3 in: A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768.

Technical Details

PALM was achieved using a custom wide-field microscope platform with 100x NA 1.4 objective lens or 150X TIRF NA 1.45 lens, yielding a pixel size at the CCD camera of 0.0792 or 0.0528 micrometers respectively. See also: A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768.

Biological Sources

NCBI Organism Classification: Drosophila melanogaster
Cell Type: early embryonic cell
Cellular Component: nuclear chromosome; chromatin

Biological Context

Biological Process: DNA packaging; chromatin organization

Attribution

Names: A Matsuda; L Shao; J Boulanger; C Kervrann; PM Carlton; P Kner; D Agard; J Sedat
Published: A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768
Pubmed: 20856676
Link: PLOSone

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39747
Archival Resource Key (ARK): ark:/b7295/w9cil39747

Grouping This image is part of a group.

Imaging

Image Type: portrayed image
Image Mode: PALM (photoactivation localization microscopy)
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP; 4',6-diamidino-2-phenylindole (DAPI)
Processing History: PALM reconstruction
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	952px	——
Y	329px	——