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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39741*  Cite 
Description

Images of negatively stained chromatin fibers prepared from tandem arrays of a nucleosome positioning sequence reconstituted with purified histones. These samples contained 80 repeats of a 167 bp DNA sequence reconstituted with core histones alone (leftmost image) plus different amounts of linker histone H5 (33%, 67%, and 100% respectively). See: Fig 3b in Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877.

Technical Details

Reconstituted chromatin was fixed with 0.1% glutaraldehyde for 30 min, deposited on glow discharged carbon films, negatively stained with aqueous uranyl acetate, and examined with a Philips 2085 TEM operated at 80 KV. See also: Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877.

Biological Sources
Cellular Component
nuclear chromatin
chromatin
Attribution
Names
Andrew Routh
Sara Sandin
Daniela Rhodes
Published
A Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877
Pubmed
18583476
Link
Proceedings National Academy of Sciences USA
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39741
Archival Resource Key (ARK)
ark:/b7295/w9cil39741
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
negative staining
uranyl salt
Processing History
montage
Data Qualifiers
processed data
Sample Preparation
Methods
glutaraldehyde fixed tissue
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 680px ——
Y 127px ——