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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39709*  Cite 
Description

Transmission electron micrograph of a yeast cell treated with 60µM sertraline. This is a high-magnification zoom of CIL 39708 showing a double-membrane autophagosome. A cresecent-shaped lipid droplet is visible, as well as multiple vesicular structures. The image contributor asks Library users to use the comments box to answer the question, what are the electron dense deposits on the surface of the lipid droplet?

Technical Details

BY4716 is an auxotrophic haploid laboratory strain of the budding yeast Saccharomyces cerevisiae. Its genotype is lys2Δ, mating type alpha and it is derived from S288c. Sections were embedded in LR white resin, mounted on carbon-coated nickel grids and imaged with LEO 912AB at 80kV and 6,300X. All cells in this batch were grown in liquid rich media (YPD pH6.5) at 30˚C with aerated shaking, and cultures were sampled in mid-logarithmic phase. This image is part of a group of untreated cells and cells exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cell Type
BY4716
Cellular Component
autophagic vacuole
autophagic vacuole membrane
lipid particle
vesicle
Biological Context
Biological Process
vacuole organization
sertraline treatement
Attribution
Names
Ethan Perlstein
Daniel Korostyshevsky
OTHER
Princeton EM Core Facility
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39709
Archival Resource Key (ARK)
ark:/b7295/w9cil39709
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in plastic embedment
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 2848px ——
Y 2311px ——