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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39334^* Cite

Description

Nuclear DNA was prepared from synchronously replicating nuclei of Physarum polycephalum, mixed with cytochrome c and formamide, and spread on the surface of a hypophase consisting of 10 mM Tris, 1 mM EDTA and 10% formamide. The surface containing the DNA-cytochrome c complexes was picked up on carbon-formvar grids, stained with ethanolic phosphotungstic acid, and rotary shadowed with Pt/Pd prior to TEM examination. Arrows point to replication forks.

Technical Details

See Fig 3A in: F Haugli et al. 1982 DNA replication in Physarum polycephalum: electron microscopic analysis patterns of DNA replication in the presence of cycloheximide. J Cell Biol 95:323-331.

Biological Sources

NCBI Organism Classification: Physarum polycephalum
Cell Type: plasmodium
Cell Line: TU291
Cellular Component: DNA

Biological Context

Biological Process: DNA replication; DNA metabolic process

Attribution

Names: Finn Haugli; Rolf Andreassen; Steiner Funderud
Published: F Haugli et al. 1982 DNA replication in Physarum polycephalum: electron microscopic analysis patterns of DNA replication in the presence of cycloheximide. J Cell Biol 95:323-331.
Pubmed: 7142290

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39334
Archival Resource Key (ARK): ark:/b7295/w9cil39334

Imaging

Image Type: film
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating; tungsten compound
Processing History: montage
Data Qualifiers: processed data

Sample Preparation

Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	420px	——
Y	1443px	——