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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39252^* Cite

Description

Mouse myoblast cells were engineered to express GFP-PCNA (proliferating cell nuclear antigen) as a marker for replication factories, and also immunostained for the DNA methylase Dnmt1. DNA was localized using Hoechst staining. The image shows two different typical S-phase patterns displaying GFP-PCNA (left), Dnmt1 (center) and DNA (right). Dnmt1 co-localizes with PCNA at replication foci.

Technical Details

C2C12 cells stably expressing GFP-PCNA were fixed,stained for DNA ligase and DNA and optical sections acquired with a Leica TCS confocal microscope. See: Fig 2 in H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: myoblast
Cell Line: C2C12
Cellular Component: nuclear chromatin; nuclear DNA

Biological Context

Biological Process: DNA replication

Attribution

Names: Heinrich Leomhardt; Hans-Peter Rahn; Peter Weinziert; Anje Sporbert; Thomas Cremer; Daniele Zink; M Cristina Cordoso
Published: H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279
Pubmed: 10769021

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39252
Archival Resource Key (ARK): ark:/b7295/w9cil39252

Grouping This image is part of a group.

Imaging

Image Type: photomultiplier tube (PMT); charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: Fluorescein (FITC); Hoechst 33258
Processing History: montage
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue; living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	396px	——
Y	279px	——