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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38716^* Cite

Description

Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were fixed, sectioned, and examined by transmission electron microscopy. Extensive regions of stacked thylakoids are seen in these wildtype cells. The section also includes the remnants of a mitochondrion (mi). See Fig 2 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619.

Technical Details

Isolated chloroplast membranes were fixed with glutaraldehyde, post fixed with osmium tetroxide, dehydrated, embedded in epoxy resin, and thin sections cut, stained and examined with a Hitachi HU-11C TEM.

Biological Sources

NCBI Organism Classification: Chlamydomonas reinhardtii
Cell Line: ac-5; ac-31
Cellular Component: chloroplast; plastid; plastid thylakoid membrane

Biological Context

Biological Process: photosynthesis; organelle organization

Attribution

Names: Ursula W. Goodenough; L. Andrew Staehelin
Published: UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619.
Pubmed: 4396088
Link: Journal of Cell Biology

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38716
Archival Resource Key (ARK): ark:/b7295/w9cil38716

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide; uranyl salt; lead salt
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in plastic embedment
Methods: microtome-sectioned tissue; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1476px	——
Y	1176px	——