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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38651^* Cite

Description

This photoactivation localization microscopy (PALM) image of tdEos-vinculin (red) and Dronpa-paxillin (green) illustrates that vinculin and paxillin are segregated into interlocking microdomains within focal adhesions. A lower magnification view that orients the adhesions on actin bundles is available as CIL 38652. Bar is 1 micron. Image made available by Catherine and James Galbraith and corresponds to Figure 5 in PNAS U S A. 2007 Dec 18;104(51):20308-13.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: fibroblast
Cell Line: foreskin fibroblast
Cellular Component: focal adhesion; vinculin; paxillin

Biological Context

Biological Process: molecular organization; focal adhesion assembly

Attribution

Names: Hari Shroff; Catherine G Galbraith; James A. Galbraith; Helen White; Jennifer Gillette; Scott Olenych; Michael W. Davidson; Eric Betzig
Published: Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 Dec 12. Erratum in: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15220.
Pubmed: 18077327
Link: article

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38651
Archival Resource Key (ARK): ark:/b7295/w9cil38651

Grouping This image is part of a group.

Imaging

Image Type: portrayed image
Image Mode: PALM (photoactivation localization microscopy)
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Dronpa; tdEos
Processing History: probability density map
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1886px	——
Y	1457px	——