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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38600^* Cite

Description

Photoactivation localization microscopy image (PALM) of a human foreskin fibroblast expressing Dronpa-actin (green) and tdEos-paxillin (red). Paxillin assembles in fibrillar-like adhesions that run parallel to ventral actin fibers and cluster near the cell periphery to form larger adhesion complexes. The differential interference contrast image (DIC) corresponding to this field is CIL 38601. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNASi U S A. 2007 Dec 18;104(51):20308-13 and corresponds to Figure 2 in PNAS U S A. 2007 Dec 18;104(51):20308-13.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: fibroblast
Cell Line: foreskin fibroblast
Cellular Component: focal adhesion; actin cytoskeleton; paxillin

Biological Context

Biological Process: molecular organization; focal adhesion assembly

Attribution

Names: Hari Shroff; Catherine G Galbraith; James A. Galbraith; Helen White; Jennifer Gillette; Scott Olenych; Michael W. Davidson; Eric Betzig
Published: Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 Dec 12. Erratum in: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15220.
Pubmed: 18077327
Link: article

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38600
Archival Resource Key (ARK): ark:/b7295/w9cil38600

Grouping This image is part of a group.

Imaging

Image Type: portrayed image
Image Mode: PALM (photoactivation localization microscopy)
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Dronpa; tdEos
Processing History: probability density map

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	7180px	——
Y	6708px	——