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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38599^* Cite

Description

This total internal reflection (TIRF) image of both the Dronpa-alphaa ctinin and the unconverted tdEos-vinculin channels corresponds to the same image field as the diffraction limited DIC image CIL 38597 and the 2-color photoactivation localization microscopy (PALM) image 38598. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNAS U S A. 2007 Dec 18;104(51):20308-13.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: fibroblast
Cell Line: foreskin fibroblast
Cellular Component: focal adhesion; actin cytoskeleton; alpha actinin; vinculin

Biological Context

Biological Process: focal adhesion assembly

Attribution

Names: Hari Shroff; Catherine G Galbraith; James A. Galbraith; Helen White; Jennifer Gillette; Scott Olenych; Michael W. Davidson; Eric Betzig
Published: Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 Dec 12. Erratum in: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15220.
Pubmed: 18077327
Link: article

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38599
Archival Resource Key (ARK): ark:/b7295/w9cil38599

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: TIRF (total internal reflection)
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Dronpa; tdEos
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	894px	——
Y	714px	——