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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36694^* Cite

Description

Zebrafish embryo undergoing cytokinesis. The embryo is expressing fixed EMTB-3GFP (microtubules-marker, green) and is labeled with phalloidin (actin marker, red) and Hoechst (DNA marker, blue). Note the radial organization of microtubules

Technical Details

Image collected on an LSM 710 using a 20X 0.8NA objective.

Biological Sources

NCBI Organism Classification: Danio rerio
Cell Type: early embryonic cell
Cellular Component: microtubule; actin filament; DNA

Biological Context

Biological Process: cytokinesis

Attribution

Names: Martin Wuehr; Timothy Mitchison

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36694
Archival Resource Key (ARK): ark:/b7295/w9cil36694

Imaging

Image Type: recorded image
Image Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein; distribution of epitope
Visualization Methods: EGFP; phalloidin; hoescht
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: methanol fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2048px	0.3459µm
Y	2048px	0.3459µm
Z	56px	2µm