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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36671^* Cite

Description

In this micrograph of a developing organelle, the trichocyst membrane is intact but does not bear the glycocalyx seen on the luminal side of the lysosomal membrane that lies nearby. The flocculant material shows no sign of crystallizing. An electron transparent zone has formed around the inside of the membrane of the largest vesicle. This cell was taken from day 2 of a culture age study. TEM taken on 3/28/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 18,000X. Bar = 0.2µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: trichocyst; organelle membrane; lysosomal membrane

Biological Context

Biological Process: organelle assembly

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36671
Archival Resource Key (ARK): ark:/b7295/w9cil36671

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2262px	——
Y	2526px	——