CIL:36012, Anura, peripheral neuron, muscle cell, myelinating Schwann cell. CIL. Dataset

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36012^* Cite

Description

Figure 394 from Chapter 15 (Cytoplasmic Inclusions) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. "Omega figures" capturing synaptic vesicle exocytosis at the synaptic junction. This event was observed during chemical stimulation of the frog neuromuscular junction, prepared by rapid freezing with liquid helium followed by freeze substition. 4-aminopyridine was used to trigger release of the neurotransmitter acetylcholine.Image by John Heuser and Tom Reese. A PDF copy of the accompanying chapter is available on the ASCB’s BioEDUCATE website.

Biological Sources

NCBI Organism Classification: Anura
Cell Type: peripheral neuron; muscle cell; myelinating Schwann cell
Cellular Component: synapse; neuromuscular junction; synaptic vesicle

Biological Context

Biological Process: synaptic vesicle exocytosis; synapse organization

Attribution

Names: Don W. Fawcett; John Heuser; Tom Reese
Published: ISBN 0721635849
Link: http://www.ascb.org/bioeducate/FawcettTheCell...

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36012
Archival Resource Key (ARK): ark:/b7295/w9cil36012

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM)
Image Mode: illumination by electrons; detection of electrons
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: osmium tetroxide
Data Qualifiers: processed data

Sample Preparation

Methods: crosslinking-fixative fixed tissue; tissue in plastic embedment; FBbi:00000621
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	895px	——
Y	1260px	——