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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35761*  Cite 
Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Clb2. Clb 2 is a cyclin-dependent protein kinase regulator, involving in G2/M transition of mitotic cell cycle and positively regulating spindle pole body separation. These phase and GFP images are part of an image group containing CIL 35760-35779. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please read the microscopy section of the referenced paper for details before image analysis.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cellular Component
CLB2 protein
spindle pole body
spindle
cellular bud neck
Attribution
Names
David Ball
Jean Peccoud
Published
PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed
22046265
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35761
Archival Resource Key (ARK)
ark:/b7295/w9cil35761
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 213.4nm
Y 512px 213.4nm
Time 300 microns 61