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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35602*  Cite 
Description

Effect of depletion of Nup133 and Nup43 by antibody addition on spindle assembly on sperm chromatin in Xenopus oocyte extract. Spindles (red) were localized by addition of tubulin-rhodamine and chromosomes stained with DAPI (blue). See Orjalo et al 2006. Mol Biol Cell 17:3806-3808.

Technical Details

Xenopus egg extracts were mixed with sperm chromatin, treated to induce spindle formation, and antibodies added to deplete nucleoporin components. Rhodamine-tubulin was added, the preparations fixed with paraformaldehyde, and spindles centrifuged onto cover slips through a glycerol cushion. Samples were examined using a Zeiss Axioskop microscope. See Fig 6B in Orjalo et al. 2006 Mol Biol Cell 17:3806-3818.

Biological Sources
NCBI Organism Classification
Xenopus laevis laevis
Cell Type
oocyte
Cellular Component
spindle
Biological Context
Biological Process
mitosis
Attribution
Names
Arturo Orjalo
Alexei Arnaoutov
Zhouxin Shen
Yekaterina Boyarchuk
Samantha Zeitlin
Beatriz Fontoura
Steven Briggs
Mary Dasso
Douglass Forbes
Published
Orjalo et al. 2006 The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Mol Biol Cell 17:3806-3818
Pubmed
16807356
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35602
Archival Resource Key (ARK)
ark:/b7295/w9cil35602
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
differences in adsorption or binding of stain
Visualization Methods
4',6-diamidino-2-phenylindole (DAPI)
Tetramethyl rhodamine (TRITC)
Processing History
montage
Data Qualifiers
processed data
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 2208px ——
Y 678px ——