Open Detailed Viewer

Licensing

This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35602^* Cite

Description

Effect of depletion of Nup133 and Nup43 by antibody addition on spindle assembly on sperm chromatin in Xenopus oocyte extract. Spindles (red) were localized by addition of tubulin-rhodamine and chromosomes stained with DAPI (blue). See Orjalo et al 2006. Mol Biol Cell 17:3806-3808.

Technical Details

Xenopus egg extracts were mixed with sperm chromatin, treated to induce spindle formation, and antibodies added to deplete nucleoporin components. Rhodamine-tubulin was added, the preparations fixed with paraformaldehyde, and spindles centrifuged onto cover slips through a glycerol cushion. Samples were examined using a Zeiss Axioskop microscope. See Fig 6B in Orjalo et al. 2006 Mol Biol Cell 17:3806-3818.

Biological Sources

NCBI Organism Classification: Xenopus laevis laevis
Cell Type: oocyte
Cellular Component: spindle

Biological Context

Biological Process: mitosis

Attribution

Names: Arturo Orjalo; Alexei Arnaoutov; Zhouxin Shen; Yekaterina Boyarchuk; Samantha Zeitlin; Beatriz Fontoura; Steven Briggs; Mary Dasso; Douglass Forbes
Published: Orjalo et al. 2006 The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Mol Biol Cell 17:3806-3818
Pubmed: 16807356

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35602
Archival Resource Key (ARK): ark:/b7295/w9cil35602

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); Tetramethyl rhodamine (TRITC)
Processing History: montage
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2208px	——
Y	678px	——