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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35598^* Cite

Description

Distribution of Bib1, BubR1 and Mad2(red) on chromosomes assembled from sperm chromatin in nocodazole treated Xenopus oocyte extracts in vitro. Chromosomes are stained with DAPI (blue). Right panels show the effect of depletion of the Nup107-160 nucleoporin complex. See Orjalo et al 2006. Mol Biol Cell 17:3806-3808.

Technical Details

Xenopus egg extracts were mixed with sperm chromatin, treated to induce spindle formation, antibodies added, and the preparations fixed with paraformaldehyde, and centrifuged onto cover slips through a glycerol cushion, processed for immunofluorescence, and examined using a Zeiss Axioskop microscope. See Fig 5A in Orjalo et al. 2006 Mol Biol Cell 17:3806-3818.

Biological Sources

NCBI Organism Classification: Xenopus laevis laevis
Cell Type: oocyte
Cellular Component: spindle; nuclear chromosome

Biological Context

Biological Process: mitosis

Attribution

Names: Arturo Orjalo; Alexei Arnaoutov; Zhouxin Shen; Yekaterina Boyarchuk; Samantha Zeitlin; Beatriz Fontoura; Steven Briggs; Mary Dasso; Douglass Forbes
Published: Orjalo et al. 2006 The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Mol Biol Cell 17:3806-3818
Pubmed: 16807356

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35598
Archival Resource Key (ARK): ark:/b7295/w9cil35598

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); Tetramethyl rhodamine (TRITC)
Processing History: montage
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1308px	——
Y	1674px	——