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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35593^* Cite

Description

Distribution of Nup133 (green) in a spindle assembled in vitro from Xenopus oocyte extracts showing localization in spindle and kinetochores. See Orjalo et al 2006. Mol Biol Cell 17:3806-3808.

Technical Details

Xenopus egg extracts were mixed with sperm chromatin, treated to induce spindle formation, antibodies added, and the preparations fixed with paraformaldehyde, and centrifuged onto cover slips through a glycerol cushion, and processed for immunofluorescence. z-stacks of images were recorded using a Zeiss Axioskop microscope with 63x !.4 NA objective lens. See Fig 3B in Orjalo et al. 2006 Mol Biol Cell 17:3806-3818.

Biological Sources

NCBI Organism Classification: Xenopus laevis laevis
Cell Type: oocyte
Cellular Component: spindle

Biological Context

Biological Process: mitosis

Attribution

Names: Arturo Orjalo; Alexei Arnaoutov; Zhouxin Shen; Yekaterina Boyarchuk; Samantha Zeitlin; Beatriz Fontoura; Steven Briggs; Mary Dasso; Douglass Forbes
Published: Orjalo et al. 2006 The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Mol Biol Cell 17:3806-3818
Pubmed: 16807356

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35593
Archival Resource Key (ARK): ark:/b7295/w9cil35593

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI)
Processing History: unprocessed raw data
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	996px	——
Y	996px	——