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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:168^* Cite

Description

The image shows a nucleus extracted from the brain of an old (29 month) rat and immuno-stained for tubulin (green)and nuclear pore complexes (red). DNA was stained with DAPI (blue). The intra-nuclear accumulation of tubulin and other cytoplasmic proteins is a property of aging cells apparently caused by deterioration of nuclear pore complexes.

Biological Sources

NCBI Organism Classification: Rattus norvegicus
Cell Type: neuron
Cellular Component: tubulin complex; nuclear envelope

Biological Context

Biological Process: cell aging

Attribution

Names: Maximilliano D'Angelo; Martin Hetzer
Published: D'Angelo et al. 2009 Cell 136:284-295.
Link: BioMedical Beat

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL168
Archival Resource Key (ARK): ark:/b7295/w9cil168

Imaging

Image Type: charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: Fluorescein (FITC); 4',6-diamidino-2-phenylindole (DAPI); Tetramethyl rhodamine (TRITC)
Processing History: montage
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	959px	——
Y	1272px	——