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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12055^* Cite

Description

Dividing cells from the liquid endosperm of the lily Haemanthus katherinae were fixed with paraformaldehye, embedded in epoxy, 0.5 micrometer sections prepared, and stained with uranyl magnesium acetate. Images were obtained with the Wisconsin high voltage TEM at 1MEV. For this low magnification survey micrograph of chromosomes at anaphase, the grid was tilted to 40 degrees. Similar micrographs tilted to 30 and 60 degrees are grouped with this image, providing oblique 3D views when used as stereo pairs.

Biological Sources

NCBI Organism Classification: Haemanthus katharinae
Cell Type: endosperm
Cellular Component: nuclear chromosome

Biological Context

Biological Process: mitosis

Attribution

Names: Hans Ris

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12055
Archival Resource Key (ARK): ark:/b7295/w9cil12055

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: elastic scattering of electrons
Visualization Methods: uranyl salt
Processing History: unprocessed raw data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5848px	4.5nm
Y	4516px	4.5nm