Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11957^* Cite

Description

Time lapse movie showing several sequences of mitosis in pollen mother cells of the Easter Lily recorded in 1951 on 16 mm film with an early polarizing microscope developed by Shinya Inoue. The work was instrumental in proving the in vivo reality of the mitotic spindle. The action is sped up by a factor of about 450x.

Technical Details

See: S. Inoue 1953. Polarization optical studies of the mitotic spindle. I. The demonstration of spindle fibers in living cells. Chromosoma 5:487-500.

Biological Sources

NCBI Organism Classification: Lilium maritimum
Cell Type: pollen mother cell
Cellular Component: spindle; nuclear chromosome

Biological Context

Biological Process: mitosis

Attribution

Names: Shinya Inoue
Published: S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL11957
Archival Resource Key (ARK): ark:/b7295/w9cil11957

Imaging

Image Type: recorded image
Image Mode: polarization microscopy
Parameters Imaged: retardance
Source of Contrast: intrinsic birefringence
Visualization Methods: visualization of contiguous regions
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	720px	——
Y	480px	——