A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged Tgalpha1tubulin and mRFP-tagged TgDLC (dynein light chain). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X, 1.4 NA. Filters: EGFP, ex 450-490 em 500-540, mRFP, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. Raw data stack corresponds to DIC data stack: CIL# 10565. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
Spatial Axis | Image Size | Pixel Size |
---|---|---|
X | 384px | 0.071µm |
Y | 384px | 0.071µm |
Z | 38px | 0.2µm |