Mouse tracheal epithelia cells (MTEC) were grown at air-liquid interface to induce differentiation, centriole amplification and ciliogenesis. Cells were fixed with ice-cold methanol for 10 min. Samples were stained with antibodies against acetylated tubulin to mark cilia (red), the centriolar protein Cep120 to mark basal bodies (green), and anti-ZO-1 to mark the cell-cell junctions (light blue). Secondary antibodies were Alexa 594 for acetylated tubulin, Alexa 488 for Cep120, and Cy5 for Zo-1. Nuclei were stained using DAPI (blue). Images were captured using Openlab 4.0.4 software controlling an Axiovert 200M microscope (Carl Zeiss, Inc.) with a 100X 1.4 NA objective.